Analytical Sciences

Interfacial-specific Chemiluminescence at Liqud/Liquid Interfaces or at O/W Emulsions Containing Lucigenin in Oil Phase

Kohji MAEDA, Hiroshi TACHIBANA, Masaki MORITA, Chika YOSHIMURA, Junichi IKEJIRI, and Yumi YOSHIDA

Department of Chemistry, Kyoto Institute of Technology, Matsugasaki, Sakyo, Kyoto 606-8585, Japan

The enhanced effect of chemiluminescence (CL) of lucigenin (Luc²⁺) at a flat liquid/liquid interface and at O/W emulsions was demonstrated. The CL was emitted when Luc²⁺ was transferred from an organic phase to an aqueous one containing NaOH and H₂O₂. The CL intensity and emission spectrum depended strongly on the kind of organic solvent. We attempted to apply the CL reaction of Luc²⁺ in the O/W emulsion system to the analysis of H₂O₂. The available analytical conditions were discussed from the aspects of the kind of organic solvent, the reaction time and wavelength detected.

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