Abstract − Analytical Sciences, 25(2), 149 (2009).
Microchip-based Homogeneous Immunoassay Using a Cloned Enzyme Dono
Tomoya TACHI,*1 Noritada KAJI,*1,*2 Manabu TOKESHI,*1,*2 and Yoshinobu BABA*1,*2,*3,*4,*5
*1 Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Nagoya 464-8603, Japan
*2 MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8603, Japan
*3 Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan
*4 Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan
*5 Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
*2 MEXT Innovative Research Center for Preventive Medical Engineering, Nagoya University, Nagoya 464-8603, Japan
*3 Plasma Nanotechnology Research Center, Nagoya University, Nagoya 464-8603, Japan
*4 Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Takamatsu 761-0395, Japan
*5 Institute for Molecular Science, National Institutes of Natural Sciences, Okazaki 444-8585, Japan
We have realized a cloned enzyme donor immunoassay (CEDIA) on a microchip in 96 s. CEDIA is a homogeneous immunoassay, based on the bacterial enzyme β-galactosidase, which was genetically engineered into two inactive fragments: an enzyme donor and an enzyme acceptor. A model analyte was theophylline, and the detectable concentration range was from 0 to 40 µg mL-1. Our CEDIA using a microfluidic device was very simple and rapid, unlike microchip-based heterogeneous immunoassays and CEDIA on a well-type microchip.
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