Analytical Sciences

Spectrophotometric Determination of Copper(II) with o-Bromophenylfluorone

Takako YAMAGUCHI, Megumi SAMMA, Shinichiro KAMINO, Momoka MATSUSHITA, Tomoyuki HASHIMOTO, and Yoshikazu FUJITA

Osaka University of Pharmaceutical Sciences, 4-20-1 Nasahara, Takatsuki, Osaka 569-1094, Japan

A simple, reliable and sensitive spectrophotometric method for the determination of copper(II) was established with o-bromophenylfluorone (OBPF), a novel chemical probe, in the presence of poly(N-vinylpyrrolidone). Beer's law was obeyed in the range of 8 − 160 ng ml⁻¹, with an apparent molar absorptivity at 570 nm, the relative standard deviation being 3.64 × 10⁵ dm³ mol⁻¹ cm⁻¹ and 0.72% (n = 5). This method was applied to the recovery tests of copper(II) in human urine, bovine serum and tap water; the results were satisfactory.

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