Analytical Sciences

Glucose Oxidase Immobilized in Alginate/Layered Double Hydroxides Hybrid Membrane and Its Biosensing Application

Shou-Nian DING,* Dan SHAN,** Huai-Guo XUE,** Dao-Bin ZHU,** and Serge COSNIER***

*School of Chemistry & Chemical Engineering, Southeast University, Nanjing 211189, P. R. China
**School of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, P. R. China
***Départment de Chimie Moléculaire UMR 5250, ICMG FR 2607, CNRS Université Joseph Fourier, BP 53, 38041 Grenoble, France

A new type of amperometric glucose biosensor based on alginate (Alg)/layered double hydroxides (LDHs) organic-inorganic composite film is described. This hybrid material combines the advantages of an organic biopolymer, Alg and inorganic LDHs. Glucose oxidase (GOD) immobilized in the material maintained its activity. The composite films were characterized by UV-Vis. The results indicated that GOD retained the essential feature of its native structure in the composite film. The Alg/LDHs/GOD-modified platinum electrode exhibited a fast response to glucose (achieve 95% of the maximum in 10 s), which provided a linear response to glucose over a concentration range of 1.6 × 10⁻⁵ − 2 × 10⁻³ M with a detection limit of 4 × 10⁻⁵ M based on S/N = 3. Furthermore, the biosensor exhibited excellent long-term stability, and satisfactory reproducibility. It retained 87% of its original activity after being used for 28 days.

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