Analytical Sciences

Capillary Chromatography Based on the Tube Radial Distribution of Aqueous-Organic Mixture Carrier Solvents: Effects of the Inner-Wall Characteristics of the Fused-Silica Tube on Separation Performance

Naoya JINNO, Ko HASHIMOTO, Masahiko HASHIMOTO, and Kazuhiko TSUKAGOSHI

Department of Chemical Engineering and Materials Science, Faculty of Science and Engineering, Doshisha University, Kyotanabe, Kyoto 610-0321, Japan

A capillary chromatography system was developed using an open capillary tube and a water-acetonitrile (hydrophilic)-ethyl acetate (hydrophobic) mixture carrier solution. In this study, we examined the effects of the inner-wall characteristics of a fused-silica capillary tube on the separation performance in this system. Untreated (silanol group-intact) and inactivated (silanol group-end-blocked) fused-silica capillary tubes were used. The mixture analyte of 1-naphthol and 2,6-naphthalenedisulfonic acid was injected into two types of capillary tubes. They were eluted from both tubes in this order with water-acetonitrile-ethyl acetate (volume ratio 2:7:4) mixture carrier solution, and eluted in reverse order with water-acetonitrile-ethyl acetate (volume ratio 15:3:2). The peak shapes observed here were analyzed while considering the inner-wall characteristics of the capillary tubes from the viewpoint of the tube radial distribution of the carrier solvents in the tubes.

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