Abstract − Analytical Sciences, 24(9), 1171 (2008).
Determination of Psoralen in Human Plasma Using Excitation-Emission Matrix Fluorescence Coupled to Second-order Calibration
A-Lin XIA, Hai-Long WU, Shao-Hua ZHU, Qing-Juan HAN, Yan ZHANG, and Ru-Qin YU
State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, China
A new algorithm, an alternating normalization-weighted error (ANWE) method, and the parallel factor analysis (PARAFAC) have been used to directly determine psoralen (PSO) in human plasma. The two methods fully exploit the second-order advantage of the applied three-way fluorescence data. Interestingly, the calibration samples need only the components of interest, and the prediction samples allow containing not only the components of interest, but also unknown interferents. Consequently, the determination of PSO in plasma becomes no longer troublesome or time-consuming. The results are satisfying. Furthermore, compared with PARAFAC, the newly introduced ANWE method can obtain more satisfactory results.
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