Abstract − Analytical Sciences, 24(8), 1025 (2008).
Zone Electrophoresis in an Inner-cooling Wide-bore Electrophoresis System with UV Detection
Yugao GUO,*,** Danning LIU,** Huaifeng WANG,** Ruijuan YUAN,** and James Jianmin BAO**
*School of Material Science and Chemical Engineering, Tianjin Polytechnic University, 63 Chenglinzhuang Road, Tianjin 300160, P. R. China
**School of Pharmaceutical Science & Technology, Tianjin University, 92 Weijin Road, Tianjin 300072, P. R. China
**School of Pharmaceutical Science & Technology, Tianjin University, 92 Weijin Road, Tianjin 300072, P. R. China
A novel, high-performance wide-bore electrophoresis (WE) system with inner-cooling has been developed. By introducing the mode of a shell and tube heat exchanger into this system to remove Joule heat generated during electrophoresis, it is feasible to extend electrophoresis from the conventional capillary (i.d. <100 µm) to a wide-bore tube (i.d. >1000 µm). The wide tube allows the loading of over 1.0 µL of the sample with an LOD of 3.0 × 10-4 mg/mL (signal-to-noise ratio, 3:1). Satisfactory separations of model compounds have been achieved on the WE system.
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