Abstract − Analytical Sciences, 24(8), 1019 (2008).
Analysis of trans-Resveratrol in Grapes by Micro-High Performance Liquid Chromatography
Enguo FAN,*1,*4 Kai ZHANG,*2 Sha JIANG,*1 Chao YAN,*3 and Yuhua BAI*1
*1 College of Life Sciences, Nankai University, Tianjin 300071, China
*2 College of Chemistry, Nankai University, Tianjin 300071, China
*3 Unimicro Technologies, Inc., 4713 First Street, Pleasanton, CA 94566, USA
*4 Institut für Pflanzen-und Zellphysiologie, Martin-Luther Universität Halle-Wittenberg, Weinbergweg 10, 06120 Halle/Saale, Germany
*2 College of Chemistry, Nankai University, Tianjin 300071, China
*3 Unimicro Technologies, Inc., 4713 First Street, Pleasanton, CA 94566, USA
*4 Institut für Pflanzen-und Zellphysiologie, Martin-Luther Universität Halle-Wittenberg, Weinbergweg 10, 06120 Halle/Saale, Germany
Resveratrol, existing in two isomeric trans-and cis-forms, is a phytoalexin that has pharmacological activities, like anti-cancer and anti-cardiovascular diseases as well as life-span increasing properties. Grapes are good sources for resveratrol introduction in the human diet not only directly, but also through its products: wine. In the present work, trans-resveratrol determination and the separation of resveratrol isomers were investigated by the use of micro-high performance liquid chromatography. The analysis conditions were optimized. The baseline separation of trans-and cis-resveratrol was obtained. Further, a trans-resveratrol determination of some grapes growing in Shacheng, Huailai, Hebei province, China was carried out with our analytical method. The concentrations were 6.77 ± 0.12 and 3.95 ± 0.18 (µg/g fresh weight) for merlot and cabernet sauvignon grapes, respectively.
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