Analytical Sciences

Amperometric Biosensor for Hydrogen Peroxide Based on Direct Electrocatalysis by Hemoglobin Immobilized on Gold Nanoparticles/1,6-Diaminohexane Modified Glassy Carbon Electrode

Mingyu TANG, Shihong CHEN, Ruo YUAN, Yaqin CHAI, Fengxian GAO, and Yi XIE

Chongqing Key Laboratory of Analytical Chemistry, College of Chemistry and Engineering, Southwest University, Chongqing 400715, P. R. China

A facile strategy of an amperometric biosensor for hydrogen peroxide based on the direct electrocatalysis of hemoglobin (Hb) immobilized on gold nanoparticles (GNPs)/1,6-diaminohexane (DAH) modified glassy carbon electrode (GCE) has been described. A uniform monolayer film of DAH was initially covalently bound on a GCE surface by virtue of the electrooxidation of one amino group of DAH, and another amino group was modified with GNPs and Hb, successively. The fabrication process was characterized by cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS) and scanning electron microscopy (SEM). The proposed biosensor exhibited an effective and fast catalytic response to the reduction of H₂O₂ with good reproducibility and stability. A linear relationship existed between the catalytic current and the H₂O₂ concentration in the range of 1.5 × 10^-6 to 2.1 × 10^-3 M with a correlation coefficient of 0.998 (n = 24). The detection limit (S/N = 3) was 8.8 × 10^-7 M.

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