Abstract − Analytical Sciences, 24(2), 293 (2008).
Phospholipid-linked Coumarin: A Fluorescent Probe for Sensing Hydroxyl Radicals in Lipid Membranes
Nobuaki SOH, Koji MAKIHARA, Tomoyuki ARIYOSHI, Daisuke SETO, Tomoharu MAKI, Hizuru NAKAJIMA, Koji NAKANO, and Toshihiko IMATO
Department of Applied Chemistry, Graduate School of Engineering, Kyushu University, 744 Moto-oka, Nishi, Fukuoka 819-0395, Japan
A fluorescent probe, DPPEC (1,2-dipalmitoylglycerophosphorylethanolamine labeled with coumarin) was developed for detecting hydroxyl radical (·OH) in lipid membranes. The coumarin moiety contributes to the fluorescent detection of ·OH and the phospholipids moiety gives a driving force to localize the probe in lipid membranes. DPPEC in liposomal membranes rapidly reacted with ·OH and increased the fluorescence intensity, depending on the concentration of ·OH. The increase in the fluorescence intensity induced by ·OH was effectively suppressed by the addition of DMSO. The probe exhibited a higher fluorescence response to ·OH over other reactive oxygen species, such as hydrogen peroxide, nitric oxide, peroxynitrite, alkylperoxyl radical, and hypochlorite. DPPEC would be useful as a new type of fluorescent probe that can localize in lipid membranes and detect ·OH efficiently.
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