Analytical Sciences

Measurement of Benzophenones in Human Urine Samples by Stir Bar Sorptive Extraction and Thermal Desorption-Gas Chromatography-Mass Spectrometry

Migaku KAWAGUCHI,* Rie ITO,* Hidehiro HONDA,* Naoyuki ENDO,* Noriya OKANOUCHI,* Koichi SAITO,* Yasuo SETO,** and Hiroyuki NAKAZAWA*

*Department of Analytical Chemistry, Faculty of Pharmaceutical Sciences, Hoshi University, 2-4-41 Ebara, Shinagawa, Tokyo 142-8501, Japan
**National Research Institute of Police Science, 6-3-1 Kashiwanoha, Kashiwa, Chiba 277-0882, Japan

Determination of benzophenones (BPs) in human urine samples by stir bar sorptive extraction (SBSE) and thermal desorption (TD)-gas chromatography-mass spectrometry (GC-MS) is described. As analytes, BP, its metabolites benzhydrol (BP-OH) and 2-hydroxybenzophenone (2OH-BP), and its derivatives 2-hydroxy-4-methoxybenzophenone (BP-3) and 2-hydroxy-4-methoxy-4′-methylbenzophenone (BP-10) were selected. After enzymatic hydrolysis, a polydimethylsiloxane (PDMS) stir bar was placed in a urine sample diluted 1:1 with water and stirred for 60 min at room temperature. The limit of quantification (LOQ) of BPs is 0.2 - 0.5 ng ml^-1 (ppb). The method showed linearity over the calibration range (0.2 - 10 or 0.5 - 10 ng ml^-1), and the correlation coefficients were equal to or higher than 0.993 for all of the analytes. The average recoveries of BPs were equal to or higher than 98.7% (RSD: 1.5 - 4.8%, n = 6).

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