Analytical Sciences

Abstract − Analytical Sciences, 24(1), 55 (2008).

Newly Developed Chemical Probes and Nano-Devices for Cellular Analysis
Aki HONDA,*1,*2 Hirokazu KOMATSU,*2 Dai KATO,*1,*3 Akio UEDA,*2,*3 Kenichi MARUYAMA,*2 Yuzuru IWASAKI,*1,*4 Takeshi ITO,*1,*5 Osamu NIWA,*1,*3 and Koji SUZUKI*1,*2
*1 Core Research for Evolutional Science and Technology (CREST) of Japan Science and Technology (JST), 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan
*2 Department of Applied Chemistry, Keio University, 3-14-1 Kohoku, Yokohama, Kanagawa 223-8522, Japan
*3 National Institute of Advanced Industrial Science and Technology, 1-1-1 Higashi, Tsukuba, Ibaraki 305-8566, Japan
*4 NTT Microsystem Integration Labs., 3-1 Morinosato Wakamiya, Atsugi, Kanagawa 243-0198, Japan
*5 Kanagawa Industrial Technology Research Institute (KITRI), 705-1 Shimo-imaizumi, Ebina, Kanagawa 243-0345, Japan
A cellular analyzing system including a “real-time cellular imaging system” and a “comprehensive analyzing system for cellular responses” was developed. A “real-time cellular imaging system” is a system used to measure real-time imaging of multiple phenomena of a single cell with high special and temporal resolutions for the purpose to understand the pathology and physiology in a single cell and realize to single cell level diagnosis. A “real-time cellular imaging system” includes multi-probe imaging with AFM (atomic force microscopy), optical and SECM (scanning electrochemical microscopy) modes, which provides us with topological information and biochemical reactions at the local area of the interior and exterior of a cell. Scanning electrochemical/optical microscopy was applied to image PC12 cells. On the other hand, cells respond to their specific substances via their ligands. Therefore, the comprehensive analysis of protein-protein interaction is the important issue to determine the functions of cells. For this purpose, a “comprehensive analysis system for cellular responses” was developed. This system is based on SPR (surface plasmon resonance) and MS (mass spectrometry) using a nano-fabricated substrate. The interaction between IL-1β and anti-IL-1β antibodies was detected.