Analytical Sciences

Synthesis of Mercaptoethylamine-coated CdSe/CdS Nanocrystals and Their Use for DNA Probe

Ping ZHONG,* Ying YU,** Jianzhong WU,** Zhaoyang LONG,*** and Chunsui LIANG***

*Department of Biology and Chemistry, Zhanjiang Educational Institute, Zhanjiang 524037, P. R. China
**Department of Chemistry and Environment, South China Normal University, Guangzhou 510006, P. R. China
***Center for Disease Control and Prevention of Guangdong Province, Guangzhou 510300, P. R. China

CdSe/CdS nanocrystals (NCs) have been synthesized in aqueous solution by using mercaptoethylamine as a stabilizer. The results of TEM, UV-Vis and FL spectra show that the product is of excellent crystal structure, uniform in radius, with extraordinary fluorescence characters. These CdSe/CdS NCs allow the ultrasensitive quantitative detection of DNA. Under the optimum conditions, linear relationships have been found between the relative fluorescence intensity and the DNA concentration in the ranges 0 - 10 and 10 - 100 µg mL^-1; the linear equations are ΔF = 210 + 80.91C and ΔF = 946 + 10.57C (C, µg mL^-1), respectively. The detection limits are 0.251 and 1.920 µg mL^-1, correspondingly. The proposed method has been applied to the determination of DNA in pig liver. It is indicated that these NCs could become a newly kind of DNA probe. In addition, the mechanism of the binding reaction has also been explored. It is considered that the binding reaction is mainly due to static electricity forces.

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