Analytical Sciences

Solid Phase Extraction of Phosphorus-containing Amino Acid-type Herbicides and Their Metabolites from Human Blood with Titania for Determination by Capillary Electrophoresis

Tetsuya ISHIWATA,* Chiho ISHIJIMA,** Akira OHASHI,** Haruki OKADA,* and Kousaburo OHASHI**

*Scientific Investigation Laboratory, Ibaraki Prefectural Police Headquarters, 978-6 Kasaharacho, Mito, Ibaraki 310-8550, Japan
**Department of Environmental Sciences, Faculty of Science, Ibaraki University, 2-1-1 Bunkyo, Mito, Ibaraki 310-8512, Japan

Phosphorus-containing amino acid-type herbicides (PAAHs) and their metabolites in human plasma and whole blood were extracted with titania and determined by capillary electrophoresis (CE). The recoveries of glyphosate (GLYP), aminomethylphosphonic acid (AMPA), gluphosinate (GLUF), and 3-methylphosphonico-propionic acid (MPPA) from human plasma were 84.6, 76.8, 90.4, and 89.6%, respectively. The recoveries of GLYP, AMPA, GLUF, and MPPA from whole blood were 79.6, 84.4, 36.9, and 31.8%, respectively. The low recoveries of GLUF and MPPA from whole blood were improved by the dilution of whole blood with water to 4-fold.

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