Analytical Sciences

A New Spectrofluorometric Probe for the Determination of Trace Amounts of CoA in Injection, Human Serum and Pig Livers

Qian PENG, Xiaoxia GE, and Chongqiu JIANG

Department of Chemistry, Shandong Normal University, Jinan 250014, China

A new spectrofluorometric method has been developed for the determination of trace amounts of coenzyme A (CoA). Using europium (Eu³⁺)-tetracycline (TC) complex as a fluorescent probe in the buffer solution of pH 6.80, CoA could remarkably enhance the fluorescence intensity of the Eu³⁺-TC complex at λ = 612 nm after adding H₅IO₆ and the enhanced fluorescence intensity is in proportion to the concentration of CoA. Optimum conditions for the determination of CoA were also investigated. The dynamic range for the determination of CoA is 6.08 × 10^-8 - 1.84 × 10^-5 mol L^-1 with detection limit of 4.62 × 10^-8 mol L^-1. This method is simple, practical and relatively free of interference from coexisting substances and can be successfully applied to determination of CoA in injection, human serum and pig liver samples. Moreover, the enhancement mechanisms of the fluorescence intensity in the Eu³⁺-TC system and the CoA-Eu³⁺-TC system have been also discussed.

J-STAGE: View this article in J-STAGE