Analytical Sciences

Abstract − Analytical Sciences, 23(4), 475 (2007).

Enhanced Electrochemical Detection of Ketorolac Tromethamine at Polypyrrole Modified Glassy Carbon Electrode
Padmanabhan SANTHOSH,* Nagarajan SENTHIL KUMAR,* Murugesan RENUKADEVI,* Anantha Iyengar GOPALAN,* Thiyagarajan VASUDEVAN,* and Kwang-Pill LEE**
*Department of Industrial Chemistry, Alagappa University, Karaikudi-630 003, India
**Advanced Analytical Science and Nanomaterials Lab, Department of Chemistry Education, Kyungpook National University, Daegu 702-701, South Korea
A glassy carbon electrode modified with a coating of polypyrrole (Ppy) exhibited an attractive performance for the detection and determination of a non-steroidal and non-narcotic analgesic compound, ketorolac tromethamine (KT). Cyclic voltammetry, differential pulse and square wave voltammetry were used in a combined way to identify the electrochemical characteristics and to optimize the conditions for detection. For calibrating and estimating KT, square-wave voltammetry was mainly used. The drug shows a well-defined peak at -1.40 V vs. Ag/AgCl in the acetate buffer (pH 5.5). The existence of Ppy on the surface of the electrode gives higher electrochemical active sites at the electrode for the detection of KT and preconcentrate KT by adsorption. The square-wave stripping voltammetric response depends on the excitation signal and the accumulation time. The calibration curve is linear in the range 1 × 10-11 to 1 × 10-7 M with a detection limit of 1.0 × 10-12 M. Applicability to serum samples was also demonstrated. A detection limit of 1.0 ng ml for serum was observed. Square-wave voltammetry shows superior performance over UV spectroscopy and other techniques.