Abstract − Analytical Sciences, 23(3), 321 (2007).
Time-Resolved Fluoroimmunoassay for 19-Nortestosterone Residues in Aquaculture Tissues
Xiangqian LI,* Tieming HUO,** Xiaogang CHU,** and Chuanlai XU**
*Department of Chemical and Bioengineering, Huaiyin Institute of Technology, 223001, Huai'an, JiangSu Province, China
**School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China
**School of Food Science and Technology, Southern Yangtze University, 214036, WuXi, JiangSu Province, China
A competitive time-resolved fluoroimmunoassay (TR-FIA) was developed for the determination of 19-nortestosterone (17β-NT) residues in aquaculture tissues. The limit of detection (LOD) was determined to be 0.08 ng g-1 and the limit of quantification (LOQ) was less than 0.8 ng g-1. The results obtained by the TR-FIA and ELISA showed a good correlation. The established TR-FIA was validated for the determination of incurred aquaculture tissues and confirmed by liquid chromatography tandem mass spectrometry (LC/MS/MS). This proposed technique could be applied to routine residue analysis.
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