Analytical Sciences

A Direct Electrochemical Biosensing Platform Constructed by Incorporating Carbon Nanotubes and Gold Nanoparticles onto Redox Poly(thionine) Film

Hui FENG,*^,** Hua WANG,* Yun ZHANG,* Bani YAN,* Guoli SHEN,* and Ruqin YU*

*State Key Laboratory of Chemo/Biosensing and Chemometrics, College of Chemistry and Chemical Engineering, Hunan University, Changsha 410082, P. R. China
**Department of Chemistry and Chemical Engineering, Hunan Act and Science University, Changde 415000, P. R. China

A direct electrochemical biosensing platform has been fabricated by covalent incorporation of carbon nanotubes (CNT) and gold nanoparticles (GNP) onto the poly(thionine) (PTH) film deposited by electropolymerization. With the synergic effects of the composite nanomaterials together with the excellent mediating redox polymer, the proposed platform could allow for faster electron transfer and higher enzyme immobilization efficiency than the platforms designed by using CNT or GNP alone. Comparison studies indicated that the as-developed H₂O₂ sensor could show greatly improved performances of amperometric responses.

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