Analytical Sciences

Ferrocenylnaphthalene Diimide-based Electrochemical Ribonuclease Assay

Masanori KANAZAWA,*^,** Shinobu SATO,*** Keiichi OHTSUKA,* and Shigeori TAKENAKA*

*Department of Applied Chemistry, Kyushu Institute of Technology, Kitakyushu, Fukuoka 840-8550, Japan
**Laboratory Water Division, Nihon Millipore K. K., Hodogaya, Yokohama 240-0005, Japan
***Department of Bioscience and Bioinformatics, Kyushu Institute of Technology, Iizuka, Fukuoka 820-8502, Japan

Messenger RNA (mRNA) poly(A)⁺RNA (from mouse kidney) was immobilized on a N-hydroxysuccinimide(NHS)-activated carboxylic acid modified electrode prepared by the treatment of a gold electrode with 3,3′-dithiodipropionic acid, followed by NHS and 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC). An electrochemical measurement using this mRNA electrode was carried out in an electrolyte containing ferrocenylnaphthalene diimide (1), and showed an electrochemical signal based on 1 concentrated on immobilized mRNA. After treating this electrode with water containing varied amounts of ribonuclease A (RNase A), the current peak based on 1 decreased with increasing in the amount of RNase A with a linear correlation in the range of 0.2 - 10 pg of RNase A.

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