Abstract − Analytical Sciences, 23(11), 1347 (2007).
Analysis of Monosaccharides by Capillary Electrophoresis with Electrochemiluminescence Detection
Ming LI* and Sang Hak LEE**
*Department of Chemistry, Pohang University of Science and Technology, Pohang 790784, Korea
**Department of Chemistry, Kyungpook National University, Taegu 702701, Korea
**Department of Chemistry, Kyungpook National University, Taegu 702701, Korea
A capillary electrophoresis with tris(2,2′-bipyridyl)ruthenium(II)-based electrogenerated chemiluminescence detection method was investigated for the determination of monosaccharides using 2-diethylaminoethanethiol as a derivatizing reagent. Xylose, rhamnose, glucose and glucosamine were selected for the demonstration of the method. Under optimal conditions, the calibration curve of glucose was linear over the range of 1.0 × 10-4 to 1.0 × 10-7 mol/L (R = 0.999) and the detection limit was 6.0 × 10-8 mol/L (S/N = 3). The method was successfully applied for the assay of glucose in angelica.
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