Analytical Sciences

Evaluation of Behavioral Differences between Native Polycyclic Aromatic Hydrocarbons and ¹³C-Labeled Internal Standards during Clean-up Steps of Analysis

Nobuyasu ITOH, Masahiko NUMATA, and Takashi YARITA

National Metrology Institute of Japan (NMIJ), National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Umezono, Tsukuba, Ibaraki 305-8563, Japan

We confirmed that concentrations of polycyclic aromatic hydrocarbons (PAHs) obtained by isotope dilution mass spectrometry (IDMS) using the corresponding ¹³C-labeled internal standards are reliable even after clean-up steps. Change in the ratio of phenanthrene to ¹³C₆-phenanthrene was less than 0.2%, although the recovery yield of ¹³C₆-phenanthrene decreased to 60%. Since changes in the ratios were commonly within the relative standard deviations of the concentrations (1.5 - 5.4%) obtained using gas chromatography-mass spectrometry in combination with pressurized-liquid extraction, concentrations obtained by IDMS with ¹³C-PAHs should be reliable.

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