Analytical Sciences

MPAI (Mass Probes Aided Ionization) Method for Total Analysis of Biomolecules by Mass Spectrometry

Aki HONDA,*^,** Shinichiro HAYASHI,* Hiroki HIFUMI,* Yuya HONMA,* Noriyuki TANJI,* Naoko IWASAWA,* Yoshio SUZUKI,* and Koji SUZUKI*^,**

*Department of Applied Chemistry, Keio University, 3-14-1 Hiyoshi, Kohoku, Yokohama, Kanagawa 223-8522, Japan
**CREST of Japan Science and Technology Agency, Kawaguchi Center Building, 4-1-8 Honcho, Kawaguchi, Saitama 332-0012, Japan

We have designed and synthesized various mass probes, which enable us to effectively ionize various molecules to be detected with mass spectrometry. We call the ionization method using mass probes the “MPAI (mass probes aided ionization)” method. We aim at the sensitive detection of various biological molecules, and also the detection of bio-molecules by a single mass spectrometry serially without changing the mechanical settings. Here, we review mass probes for small molecules with various functional groups and mass probes for proteins. Further, we introduce newly developed mass probes for proteins for highly sensitive detection.

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