Analytical Sciences

Onboard Determination of Submicromolar Nitrate in Seawater by Anion-Exchange Chromatography with Lithium Chloride Eluent

Masahiro MARUO,* Takashi DOI,** and Hajime OBATA**

*School of Environmental Science, The University of Shiga Prefecture, Hassaka, Hikone, Shiga 522-8533, Japan
**Ocean Research Institute, The University of Tokyo, Minamidai, Nakano, Tokyo 164-8653, Japan

Ion-exchange chromatography using a high-capacity anion exchanger with UV detection was applied to the determination of nitrate in seawater. Major ions in seawater samples did not affect the peak shape and the retention time of the nitrate when an alkaline metal cation-chloride solution was used as an eluent at high concentrations (0.5 - 2 mol/l). At a wavelength of 220 nm, the peak of bromide was very small because of low absorption, while its separation from the nitrate peak was good at high concentrations. Among the eluents tested, lithium chloride gave the best separation of nitrate from bromide. It was estimated that the lithium ion had the least potential for ion-pair formation with nitrate, and its retention time was prolonged compared with the retention times when using other cations; with bromide and nitrite, such an effect was not observed. The results of shipboard seawater nitrate determination by our method in the South Pacific Ocean and Antarctic Sea showed good agreement with those by the conventional photometric method using continuous flow.

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