Analytical Sciences

Analysis of the Difference in Color Development in the Dye-binding Method Due to the Kind of Buffer Solution

Yuji SUZUKI

Department of Medical Technology, Saitama Prefectural University Junior College, 820, Sannomiya, Koshigaya, Saitama 343-8540, Japan

In a dye-binding method using a pH indicator, color development has reportedly been affected by the kind of buffer solution used in the color reagent. This phenomenon was analyzed by using a calculation based on the assumption that the anion of the buffer solution also reacts with protein. Color development decreases with increases in the anion concentration of the buffer solution and in the equilibrium constant of the reaction between the anion and protein. The differences in color development due to the kind of buffer solution can be attributed to differences in the equilibrium constant of the reaction forming the anion-protein complex and to the concentration of the anion between the buffer solutions.

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