Abstract − Analytical Sciences, 22(6), 815 (2006).
Simple and Rapid Determination of Hydrogen Peroxide Using Phosphine-based Fluorescent Reagents with Sodium Tungstate Dihydrate
Maki ONODA, Takefumi UCHIYAMA, Ken-ichi MAWATARI, Kiyoko KANEKO, and Kazuya NAKAGOMI
Department of Analytical Chemistry, School of Pharmaceutical Sciences, Teikyo University, Kanagawa 199-0195, Japan
A simple batch method for the fluorometric determination of hydrogen peroxide using phosphine-based fluorescent reagents has been developed. A rapid, mild and selective derivatization reaction was achieved by adding sodium tungstate dihydrate to the reaction mixture of hydrogen peroxide and a phosphine-based fluorescent reagent. When 4-diphenylphosphino-7-methylthio-2,1,3-benzoxadiazole was used as a reagent, the derivatization reaction was completed after 2 min at room temperature. The calibration curve was linear between 12.5 and 500 ng hydrogen peroxide in a 10 µL sample solution. This method is accurate and has potential for on-line applications.
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