Analytical Sciences

Evaluation of Methanol-Water and Acetonitrile-Water Binary Mixtures as Eluents for Temperature-dependent Inclusion Chromatography

Pawel K. ZARZYCKI,*^,** Elzbieta WLODARCZYK,* Da-Wei LOU,** and Kiyokatsu JINNO**

*Laboratory of Toxicology, Department of Environmental Biology, Technical University of Koszalin, 75-453 Koszalin, Poland
**School of Materials Science, Toyohashi University of Technology, Toyohashi 441-8580, Japan

In the present study the solubility of β-cyclodextrin and 2-hydroxypropyl-β-cyclodextrin at sub-zero and elevated temperatures (-10 and +30°C) for a given composition of methanol/water and acetonitrile/water binary mixtures (X_s = 0.16) was studied. Moreover, the freezing temperature profiles of acetonitrile-based chromatographic mobile phases were measured, and the obtained results were compared with data available in the literature. Furthermore, the effect of the macrocycles concentration on the liquid-phase freezing points was determined. The low solubility of native β-cyclodextrin in a methanol/water mixture at sub-zero temperature as well as the non-linear behavior of acetonitrile/water mixtures that were observed concerning the freezing point profile are discussed from a practical point of view.

J-STAGE: View this article in J-STAGE