Analytical Sciences

Indirect Fluorometric Determination of Diclofenac Sodium

Marcela A. CASTILLO*^,** and Liliana BRUZZONE*

*División Química Analítica, Facultad de Ciencias Exactas, Universidad Nacional de La Plata, Calle 47 esq. 115, 1900 La Plata, Argentina
**CIDEPINT, 52 e/121 y 122, 1900 La Plata, Argentina

A simple and easy method of analysis for diclofenac sodium is reported. A spectrofluorometric method for the microdetermination of diclofenac sodium has been developed through its reaction with cerium(IV) in an acidic solution and measurement of the fluorescence of the Ce(III) ions produced. Under the optimum experimental conditions for the oxidation reaction, 1.0 M H₂SO₄ with 90 min of heating time (100°C), the range of application is 124.3 - 600 ng mL^-1 and the limit of detection is 72.7 ng mL^-1. The proposed method was applied to the determination of diclofenac sodium in pharmaceutical tablets. The results of the analysis show a good agreement with those obtained by the official USP 27 HPLC method.

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