Analytical Sciences

Abstract − Analytical Sciences, 22(3), 393 (2006).

Simultaneous Electrochemical Determination of Uric Acid and Ascorbic Acid on a Glassy Carbon Electrode Modified with Cobalt(II) Tetrakisphenylporphyrin
Chun-Xiang LI,* Yun-Long ZENG,** Ying-Ju LIU,** and Chun-Rang TANG*
*Chemistry and Chemical Engineering College, Hunan University of Science and Technology, Xiangtan 411201, P. R. China
**State Key Laboratory of Chemo/Biosensing and Chemometrics, Chemistry and Chemical Engineering College, Hunan University, Changsha 410082, P. R. China
A cobalt(II) tetrakisphenylporphyrin (Co(II)TPP) film modified glassy carbon electrode (Co(II)TPP-GCE) was prepared by just coating Co(II)TPP solution on the surface of the electrode. It can be used for the simultaneous determination of ascorbic acid and uric acid. The anodic peaks of AA and UA can be separated well. Owing to the strongly hydrophobic property of porphyrin, the modified electrode has good stability and long life. The linear range for UA and AA were 2.0 × 10-6 - 1.0 × 10-4 M and 9.0 × 10-6 - 2.0 × 10-3 M with detection limits of 5.0 × 10-7 and 5.0 × 10-6 M, respectively. Furthermore, metalloporphyrins of other kinds were also used to construct modified electrodes. Their performances were inferior compared with that of the Co(II)TPP modified electrode.