Analytical Sciences

Pyraflufen-ethyl Residues in Soil by Solid Phase Extraction and High-Performance Liquid Chromatography with UV Detection

Hui-Li WANG, Jian-Zhong LI, and Ji-Ye HU

Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing, 100085, China

Solid-phase extraction (SPE) procedure for cleanup followed by HPLC-UV method has been investigated for the determination of pyraflufen-ethyl residues in soil. The pesticide is extracted from the sample with acetone-water (80:20, v/v) and the extract is loaded onto an octadecyl (C₁₈) column. The pesticide is eluted with acetonitrile and determined by HPLC with a UV detector. Using an acetone-water extraction followed by a C₁₈ cleanup, this method is characterized by recovery >90.1%, precision <5.8% RSD and sensitivity of 0.01 mg/kg. The proposed method has been successfully employed for the determination of the degradation dynamics of pyraflufen-ethyl in four agricultural soil samples under laboratory conditions.

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