Abstract − Analytical Sciences, 21(6), 697 (2005).
Determination of Bisphenol-A in Water by Semi-Micro Column High-Performance Liquid Chromatography Using 2-Methoxy-4-(2-phthalimidinyl)-phenylsulfonyl Chloride as a Fluorescent Labeling Reagent
Yasuto TSURUTA,* Hirofumi INOUE,* Keiko FUKUNAGA,* Sayaka MUNEMURA,* Misa OZAKI,* Masaya OHTA,** and Fumito MATSUURA**
*Faculty of Pharmacy and Pharmaceutical Sciences, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan
**Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan
**Department of Biotechnology, Faculty of Life Science and Biotechnology, Fukuyama University, Fukuyama, Hiroshima 729-0292, Japan
A highly sensitive method for the determination of bisphenol-A in water with semi-micro column high-performance liquid chromatography using 2-methoxy-4-(2-phthalimidinyl)phenylsulfonyl chloride as a fluorescent labeling reagent has been developed. The labeling reaction was carried out at 70°C for 20 min in borate buffer (pH 9.5). The derivative eluted at 11.6 min on a reversed-phase column with methanol-water (78:22, v/v) at a flow-rate of 0.2 ml/min. The fluorescence was monitored at 308 nm for excitation and 410 nm for emission. The detection limit (S/N = 3) was 10 fmol per injection. The labeling yield was about 95%.
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