Analytical Sciences

Abstract − Analytical Sciences, 21(5), 565 (2005).

Simultaneous Determination of N,N-Dimethylaniline and Phenol in Wastewater by High-Performance Liquid Chromatography with Chemiluminescence Detection
Cheng HUANG, Guangming ZHOU, Hongjun PENG, and Zhixi GAO
School of Chemistry and Chemical Engineering, Southwest Normal University, Chongqing 400715, P. R. China
A method using HPLC-CL linkage was developed for simultaneous determination of N,N-dimethylaniline and phenol in wastewater, based on the strong sensitive chemiluminescence of the luminol-K3Fe(CN)6 systems in alkaline medium. The separation was carried out on a Hypersil ODS column with a mobile phase of ethanol-0.01% triethylamine (2:1, v/v). The linear ranges for N,N-dimethylaniline determinations were 2.0 × 10-7 - 2.5 × 10-5 g/mL and 4.0 × 10-5 - 1.5 × 10-4 g/mL with a detection limit (3 σ) of 1.20 × 10-8 g/mL; the relative standard deviation (3 σ) for 5.0 × 10-6 g/mL N,N-dimethylaniline was 1.4% (n = 6). The range for phenol was from 5.1 × 10-7 to 1.3 × 10-4 g/mL, and a detection limit (3 σ) of 2.5 × 10-8 g/mL could be obtained. The method can be useful for the determination of N,N-dimethylaniline and phenol in some environmental samples.