Analytical Sciences

Electroanalysis of Tetracycline Using Nickel-implanted Boron-doped Diamond Thin Film Electrode Applied to Flow Injection System

Surudee TREETEPVIJIT,* Suchada CHUANUWATANAKUL,* Yasuaki EINAGA,** Rika SATO,** and Orawon CHAILAPAKUL*

*Department of Chemistry, Faculty of Science, Chulalongkorn University, Patumwan, Bangkok 10330, Thailand
**Department of Chemistry, Faculty of Science and Technology, Keio University, 3-14-1 Hiyoshi, Yokohama, Kanagawa 223-8522, Japan

The electrochemical analysis of tetracycline was investigated using nickel-implanted boron-doped diamond thin film electrode by cyclic voltammetry and amperometry with a flow injection system. Cyclic voltammetry was used to study the electrochemical oxidation of tetracycline. Comparison experiments were carried out using as-deposited boron-doped diamond thin film electrode (BDD). Nickel-implanted boron-doped diamond thin film electrode (Ni-DIA) provided well-resolved oxidation irreversible cyclic voltammograms. The current signals were higher than those obtained using the as-deposited BDD electrode. Results using nickel-implanted boron-doped diamond thin film electrode in flow injection system coupled with amperometric detection are presented. The optimum potential for tetracycline was 1.55 V versus Ag/AgCl. The linear range of 1.0 to 100 µM and the detection limit of 10 nM were obtained. In addition, the application for drug formulation was also investigated.

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