Analytical Sciences

Simultaneous Detection of Monoethanolamine, Diethanolamine, and Triethanolamine by HPLC with a Chemiluminescence Reaction and Online Derivatization to Tertiary Amine

Nobumitsu NIINA,* Hitoshi KODAMATANI,** Kayoko UOZUMI,* Yuki KOKUFU,* Keiitsu SAITO,** and Shigeo YAMAZAKI*

*Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan
**Division of Natural Environment and Chemistry, Faculty of Human Development, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan

In this paper we propose a new postcolumn detection method for compounds having primary, secondary, and tertiary amine moieties. The primary and secondary amine are delivatized by a reaction with epichlorohydrin having an epoxy moiety in a reaction coil to yield a tertiary amine with subsequent chemiluminescence detection using [Ru(bpy)₃]³⁺. The liner values of the calibration curves of monoethanolamine (MEA), diethanolamine (DEA) and triethanolamine (TEA) were 0.02 - 1.0 nmol (r² = 0.9986), 0.02 - 0.5 nmol (r² = 0.9993) and 0.1 - 1.0 nmol (r² = 0.9482), respectively. Also, the detection limits (S/N = 3) of MEA, DEA and TEA were 30, 25 and 40 pmol, respectively. The amount of DEA and TEA in shaving cream (60 µg/20 µL) were found to be 0.3 nmol and 14 nmol, respectively, by the proposed method.

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