Analytical Sciences

Droplet-based Micro-flow Chemiluminescence System for in vivo Glucose Determination by Microdialysis Sampling

Wei LIU,*^,** Zhujun ZHANG,*^,** and Haisheng LIU**

*Department of Chemistry, Institute of Analytical Science, Southwest Normal University, Beibei, Chongqing 400715, P. R. China
**School of Chemistry and Materials Science, Shaanxi Normal University Xi’an, 710062, P. R. China

A micro-flow chemiluminescence (CL) system in vivo for glucose determination by the on-line microdialysis sampling is described in this paper. The micro-flow CL system uses discrete sample droplets, which formed at the tip of the capillary with the sampling volume of 4.5 µl. The sol-gel method is introduced to co-immobilize horseradish peroxidase (HRP) and glucose oxidase (GOD) on the inside surface of the micro-flow cell which was fabricated in polymethyl methacrylate (PMMA). The CL detection involved enzymatic oxidation of glucose to D-gluconic acid and H₂O₂, then H₂O₂ oxidizing luminol to produce CL in presence of HRP. The microdialysis probe was utilized for sampling in the rabbit blood; the sample throughput was 20 h^-1. The glucose level in blood of the rabbit was on-line monitored with good results.

J-STAGE: View this article in J-STAGE