Analytical Sciences

Determination of Oxycodone and Hydrocotarnine in Cancer Patient Serum by High-Performance Liquid Chromatography with Electrochemical Detection

Hideya KOKUBUN,*¹ Makiko OUKI,*² Motohiro MATOBA,*³ Hiroaki KUBO,*⁴ Sumio HOKA,*³ and Kazuo YAGO*¹

*1 Department of Pharmacy, Kitasato University Hospital, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan
*2 Department of Pharmacy, St. Marianna University School of Medicine Hospital, 2-16-1, Sugao, Miyamae, Kawasaki, Kanagawa 216-8511, Japan
*3 Department of Anesthesiology, Kitasato University School of Medicine, 1-15-1, Kitasato, Sagamihara, Kanagawa 228-8555, Japan
*4 School of Pharmaceutical Sciences, Kitasato University, 5-9-1, Shirokane, Minato, Tokyo 108-8641, Japan

We developed an HPLC procedure using electrochemical detection for the quantitation of oxycodone and hydrocotarnine in cancer patients serum. An eluent of methanol:acetonitrile:5 mM pH 8 phosphate buffer (2:1:7) was used for the mobile phase. The calibration curve was linear in the range from 10 ng/mL to 100 ng/mL. The recovery of oxycodone and hydrocotarnine was 97.2% and 90.5%, respectively. The relative standard deviations within-runs and between-runs for the assay of oxycodone or hydrocotarnine were less than 4.8%. The method developed here was better than the method reported previously.

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