Analytical Sciences

Ultrafast Diagnosis of the Genetic-related Disorders Using the Combined Technologies of Multiplex PCR and Multichannel Microchip Electrophoresis

Mohammad JABASINI,* Ashraf A. EWIS,** Feng XU,* Guichen PING,* Maged FOUAD,* Toshikatsu SHINKA,*** Yutaka NAKAHORI,*** Mitsuru ISHIKAWA,** and Yoshinobu BABA*^,**

*Department of Applied Chemistry, Graduate School of Engineering, Nagoya University, Furo-cho, Chikusa-ku, Nagoya 464-8603, Japan
**Health Technology Research Center, National Institute of Advanced Industrial Science and Technology (AIST), Hayashi-cho 2217-14, Takamatsu 761-0395, Japan
***Department of Human Genetics Public Health, School of Medicine, The University of Tokushima, Tokushima 770-8505, Japan

For the diagnosis of unexplained male infertility a multiplex PCR for 6 markers, which are well-known as candidate genes for studying male infertility and located on the human Y-chromosome, has been designed. The multiplex PCR products have been separated on a 12 channel microchip electrophoresis system, which can analyze different samples simultaneously. By combining the technologies of multiplex PCR with multichannel microchip electrophoresis, the number of the DNA markers that can be screened simultaneously is increased to be 72 marker (12 × 6) in a single run while the electrophoresis analysis time is reduced to be only 180 s.

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