Analytical Sciences

Pattern Recognition Analysis for Classification of Hypertensive Model Rats and Diurnal Variation Using ¹H-NMR Spectroscopy of Urine

Masako FUJIWARA,* Kazunori ARIFUKU,* Itiro ANDO,** and Tadashi NEMOTO***

*JEOL DATUM LTD, 1156, Nakagami-cho, Akishima-shi, Tokyo 196-0022, Japan
**Environmental Research Center Ltd., 3-1, Hanari, Tsukuba-shi, Ibaraki 305-0857, Japan
***National Institute of Advanced Industrial Science and Technology (AIST), 1-1, Higashi, Tsukuba-shi, Ibaraki 305-8566, Japan

Urine samples were collected during the daytime and nighttime from spontaneously hypertensive model rats and normal rats without dosing. The ¹H NMR spectra were measured for their urine samples, and analyzed by a pattern recognition method, known as Principal Component Analysis (PCA) and Soft Independent Modeling of Class Analogy (SIMCA). The separation of urinary data due to the diurnal variation (daytime and nighttime) and also to the difference between the two strains of rat was achieved in the PCA score plot. Differences of the urinary profiles in the respective separation were effectively extracted as marker variables by the SIMCA method. NMR measurements coupled with pattern recognition methods provide a straightforward approach to inspect the disease metabolic status and the preliminary screening tool of marker candidates for further development.

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