Analytical Sciences

Estimation of Trace Amounts of Chromium(III) in Multi-Vitamin with Multi-Mineral Formulations

Jolly M. SANKALIA, Rajashree C. MASHRU, Mayur G. SANKALIA, Priti P. PARIKH, and Vijay B. SUTARIYA

Pharmacy Department, Faculty of Technology & Engineering, The Maharaja Sayajirao University of Baroda, Kalabhavan, Vadodara, Gujarat 390001, India

Two new specific, selective, simple and inexpensive spectroscopic methods for estimating a trace amount of chromium (Cr³⁺) from a multi-vitamin with multi-mineral pharmaceutical formulations were developed. The proposed methods are based on the conversion of Cr³⁺ to Cr⁶⁺ either by oxidation with a nitric acid-perchloric acid mixture (method I) or by fusion with an excess amount of sodium carbonate (method II), followed by the complexation of Cr⁶⁺ with 1,5-diphenylcarbazide (DPC) in a mineral acidic solution of pH 1.0 ± 0.5. The pink-colored complex was estimated at 544 nm. Both methods were found to be linear in the range of 0.1 - 0.8 µg/ml with a limit of detection in the range of 0.0123 - 0.0157 µg/ml and a limit of quantitation in the range of 0.0419 - 0.0525 µg/ml. Method I was found to be suitable for estimating Cr³⁺ species in various formulations, like tablets, capsules and syrups, while method II was found to be suitable for tablets and capsules. Satisfactory recovery from spiked samples of standard Cr³⁺ suggests no interference of any excipients and diverse ions present in the formulations. The developed methods were compared with AAS by ANOVA, and no significant difference was observed.

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