Abstract − Analytical Sciences, 20(8), 1127 (2004).
The Interaction of Copper-Bipyridyl Complex with DNA and Cleavage to DNA
Zhou-Sheng YANG, Yan-Ling WANG, and Guang-Chao ZHAO
School of Chemistry and Materials Science, Anhui Normal University, Wuhu 241000, P. R. China
The interaction of a copper-bipyridyl (bpy) complex with CT-DNA was investigated by voltammetry, absorption and fluorescence spectrophotometry. The binding constant of the Cu(bpy)22+ complex interacting with DNA was 3.24 × 104 L/mol and the ability binding of Cu(bpy)22+ to DNA was 1.3-times as large as that of Cu(bpy)2+ to DNA. DNA could be efficiently cleaved by a potential-modulated method in the presence of the Cu(bpy)22+ complex. The fragments of the cleaved DNA were separated by high-performance liquid chromatography (HPLC). The experimental results revealed that the proposed method for DNA cleavage is highly efficient.
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