Abstract − Analytical Sciences, 20(7), 1065 (2004).
Sensitive Determination of Domoic Acid Using High-Performance Liquid Chromatography with Electrogenerated Tris(2,2′-bipyridine)ruthenium(III) Chemiluminescence Detection
Hitoshi KODAMATANI,*1 Keiitsu SAITO,*2 Nobumitsu NIINA,*3 Shigeo YAMAZAKI,*3 Akihiko MUROMATSU,*2 and Ikuo SAKURADA*4
*1 Graduate School of Cultural Studies and Human Science, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan
*2 Division of Natural Environment and Chemistry, Faculty of Human Development, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan
*3 Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan
*4 Comet Co., 5-33-1 Kamiaso, Aso, Kawasaki 215-0021, Japan
*2 Division of Natural Environment and Chemistry, Faculty of Human Development, Kobe University, 3-11 Tsurukabuto, Nada, Kobe 657-8501, Japan
*3 Department of Applied Science, Okayama University of Science, 1-1 Ridaicho, Okayama 700-0005, Japan
*4 Comet Co., 5-33-1 Kamiaso, Aso, Kawasaki 215-0021, Japan
A new sensitive determination method of domoic acid based on the chemiluminescent reaction of tris(2,2′-bipyridine)ruthenium(III) has been developed. The method exhibited good reproducibility. The relative standard deviation of six replicate measurements was 1.6% for 10 ng ml-1. A calibration graph, based on a standard domoic acid solution, was linear over the range of 1 - 500 ng ml-1 (coefficient of correlation, r2 = 0.9995) and the detection limit was 8 pg (signal-to-noise ratio = 3) without any preconcentration and derivatization steps. This new method was successfully applied to a real sample of blue mussels spiked with 2 µg g-1 domoic acid.
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