Analytical Sciences

Spectral Properties of the Association Nanoparticle System of Ciprofloxacin-Phloxine and Its Application to Fluorescence Analysis

Jieming ZOU,* Zhiliang JIANG,** Lisheng WANG,* Tingsheng LI,** and Qinye LIU*

*Guilin Sajin Pharmceutical Company Group, Guilin 541004, China
**Department of Resource and Environmental Science, Guangxi Normal University, Guilin 541004, China

There is a fluorescence peak at 570 nm, and a maximum absorption peak at 560 nm for phloxine (PHLO) in a pH 7 water solution. Under these conditions, the ciprofloxacin cation (CPFX⁺) and PHLO^- combine into hydrophobic CPFX-PHLO association molecule by means of static gravitation. There are stronger van der Waals forces and hydrophobic forces among the CPFX-PHLO molecules. Thus, they aggregate automatically to the (CPFX-PHLO)_n association nanoparticle in red-violet color. That was characterized by scan electron microscopy (SEM), hyperfiltration and dialysis tests. In 0.04 M HCl, the red-violet nanoparticles exhibited a Rayleigh scattering peak at 470 nm, a resonance scattering peak at 580 nm, a maximum absorption wavelength at 565 nm, and a fluorescence peak at 450 nm. The fluorescence analytical conditions of CPFX have been considered. The CPFX concentration in the range of 1.0 × 10^-6 - 4.0 × 10^-5 M is linear to the fluorescence intensity, F_450nm. The detection limit was achieved at 4.0 × 10^-7 M CPFX. The CPFX in real samples was determined with satisfactory results.

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