Abstract − Analytical Sciences, 20(6), 895 (2004).
Oscillatory Reaction Using Immobilized Catalase
Satoshi SASAKI* and Yoshikazu ARAI**
*School of Bionics, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo 192-0982, Japan
**Department of Electronics, School of Engineering, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo 192-0982, Japan
**Department of Electronics, School of Engineering, Tokyo University of Technology, 1404-1, Katakura, Hachioji, Tokyo 192-0982, Japan
Oscillation is found in many biological systems, and among them the enzymatic oscillatory reaction has been well studied using an enzyme solution. We show in this study for the first time that oscillation occurs when catalase is immobilized to controlled pore glass (CPG). The oscillatory wave mode changes with the distance among the CPG, electrode, or dialysis membrane. The lower substrate concentration results in oscillation with a longer period. This tendency agrees with a previous study using an enzyme solution. Furthermore, we show that the oscillation occurs when no dialysis membrane is used. These results show the wider applicability of the system to analysis or novel device fabrication.
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