Abstract − Analytical Sciences, 20(6), 893 (2004).
Single-base Mutation Detection Using Neutravidin-modified Polystyrene Nanoparticle Aggregation
Kae SATO, Munetaka SAWAYANAGI, Kazuo HOSOKAWA, and Mizuo MAEDA
Bioengineering Laboratory, RIKEN (The Institute of Physical and Chemical Research), Hirosawa 2-1, Wako, Saitama 351-0198, Japan
A single-base mutation assay using the non-crosslinking aggregation of neutravidin-modified polystyrene nanoparticles is described. This method requires only two steps: hybridization of biotinylated probe and sample DNAs, and then mixing with neutravidin-modified nanoparticles. The aggregation was detected within 20 min in total. A combination of the DNA non-crosslinking aggregation and biotin-avidin technology has allowed sufficient performance for the detection of single nucleotide polymorphisms.
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