Abstract − Analytical Sciences, 20(3), 557 (2004).
Simultaneous Determination of Histamine and Histidine by Liquid Chromatography Following Intramolecular Excimer-forming Fluorescence Derivatization with Pyrene-labeling Reagent
Hideyuki YOSHIDA,*1 Fumio ICHINOSE,*2 Takashi YOSHITAKE,*3 Yukitaka NAKANO,*4 Kenichiro TODOROKI,*1 Hitoshi NOHTA,*1 and Masatoshi YAMAGUCHI*1
*1 Faculty of Pharmaceutical Sciences, Fukuoka University, Johnan, Fukuoka 814-0180, Japan
*2 Chemicals Evaluation and Research Institute, 3-822 Ishii Machi, Hita City, Oita 877-0061, Japan
*3 Department of Neuroscience, Karolinska Institutet, Retzius Väg 8, S-17177 Stockholm, Sweden
*4 Department of Pharmacy, Saga University Hospital, 5-1-1 Nabeshima, Saga 849-8501, Japan
*2 Chemicals Evaluation and Research Institute, 3-822 Ishii Machi, Hita City, Oita 877-0061, Japan
*3 Department of Neuroscience, Karolinska Institutet, Retzius Väg 8, S-17177 Stockholm, Sweden
*4 Department of Pharmacy, Saga University Hospital, 5-1-1 Nabeshima, Saga 849-8501, Japan
A highly sensitive and selective fluorometric method for the determination of histamine and histidine has been developed. This method is based on an intramolecular excimer-forming fluorescence derivatization with a pyrene reagent followed by reversed-phase liquid chromatography. The analytes, containing two amino moieties in a molecule, were converted to the corresponding dipyrene-labeled derivatives by derivatization. The derivatives afforded intramolecular excimer fluorescence (440 - 540 nm), which can clearly be discriminated from the normal fluorescence (360 - 420 nm) emitted from reagent blanks. The detection limits (signal-to-noise ratio = 3) were femto mole levels.
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