Analytical Sciences

Preparation and a Time-Resolved Fluoroimmunoassay Application of New Europium Fluorescent Nanoparticles

Xiaodan HAI,* Mingqian TAN,* Guilan WANG,* Zhiqiang YE,* Jingli YUAN,*  and Kazuko MATSUMOTO**

*Department of Analytical Chemistry, Dalian Institute of Chemical Physics, Chinese Academy of Sciences, Dalian 116012, P. R. China
**Department of Chemistry, Waseda University, Advanced Research Institute for Science and Engineering, Tokyo 169-8555, Japan

New silica-based europium fluorescent nanoparticles having surface amino groups were prepared by a covalent bindingcopolymerization technique. In the nanoparticles, the fluorescent Eu³⁺ chelate molecules were covalently bound to silicon atoms to protect the nanoparticles from dye leaking in bio-applications. The amino groups on the surface of nanoparticles made the surface modification and bioconjugation of nanoparticles easier. The nanoparticles were characterized and developed as a new type of fluorescence probe for a highly sensitive time-resolved fluoroimmunoassay (TR-FIA) of human hepatitis B surface antigen (HBsAg).

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