Analytical Sciences

Glucose Sensor Based on Glucose Oxidase Immobilized by Zirconium Phosphate

Sejin PARK,*^,** Taek Dong CHUNG,* Sun Kil KANG,** Ran-A JEONG,*^,** Hankil BOO,*^,** and Hee Chan KIM**

*Department of Chemistry, Sungshin Women’s University, 249-1, Dongsun-dong, Seongbuk-gu, Seoul 136-742, Korea
**Department of Biomedical Engineering, College of Medicine and Institute of Medical and Biological Engineering, Medical Research Center, Seoul National University, 28 Yongon-dong, Chongno-ku, Seoul 110-744, Korea

Amperometric glucose sensors were fabricated using glucose oxidase (GOx) entrapped in zirconium hydrogenphosphate (ZrP), and their performance was evaluated. Reportedly, α-ZrP is one of the candidates that are expected to improve the stability of enzymes immobilized on solid surfaces.^1,2 We intercalated GOxs into ZrP (GOx/ZrP), cast the GOx/ZrP suspension in polyvinylalcohol on a platinum electrode, and dried it in a vacuum oven. The morphological layered structure was investigated by scanning electron microscopy. The enzymatic activities, which were determined by open-circuit potentiometric technique, reached the highest when GOxs were immobilized in ZrP at ca. pH 5. In vitro tests showed good linear responses in the 0 - 25 mM range and the sensitivity of 0.14 nA mM^-1 at 0.4 V vs. Ag/AgCl. The sensors, as made, were stable for more than 3 days within a limited deterioration.

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