Analytical Sciences

Application of the ¹⁹F NMR Technique to Observe Binding of the General Anesthetic Halothane to Human Serum Albumin

Kazuaki SHIKII,*^1,*² Satoshi SAKURAI,*³ Hiroaki UTSUMI,*³ Hiroko SEKI,*¹ and Mitsuru TASHIRO*⁴

*1 Chemical Analysis Center, Chiba University, Yayoi, Inage, Chiba 263-8522, Japan
*2 Analysis Research Department, Chemical Research Laboratories, Nissan Chemical Industries Ltd., Tsuboi, Funabashi, Chiba 274-8507, Japan
*3 NM Application & Research Group, Application & Research Center, Analytical Instruments Division, JEOL Ltd., Musashino, Akishima, Tokyo 196-8558, Japan
*4 Department of Chemistry, Faculty of Science, Tokyo Metropolitan University, Minami-Osawa, Hachioji, Tokyo 192-0367, Japan

¹⁹F NMR techniques were employed to characterize the binding property of the widely used general anesthetic halothane with human serum albumin (HSA). It was found that ¹⁹F{¹H} NOE and 2D ¹H-¹⁹F HOESY experiments detected intermolecular NOEs between halothane ¹⁹F and HSA protons. Measurements of the diffusion coefficients for halothane were also carried out by ¹H and ¹⁹F NMR, indicating the interaction of halothane with HSA. The present results indicate that these techniques are very suitable to identify a fluorine-containing ligand binding with a protein receptor in the drug-discovery process.

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