Analytical Sciences

An Amperometric Biosensor for Glucose Based on Electrodeposited Redox Polymer/Glucose Oxidase Film on a Gold Electrode

Junjie FEI, Yunhua WU, Xiaobo JI, Jing WANG, Shengshui HU,  and Zhiqiang GAO 

Department of Chemistry, Wuhan University, Wuhan 430072, P. R. China

In this paper, we described a glucose biosensor based on the co-electrodeposition of a poly(vinylimidazole) complex of [Os(bpy)₂Cl]^+/2+ (PVI-Os) and glucose oxidase (GOX) on a gold electrode surface. The one-step co-electrodeposition method provided a better control on the sensor construction, especially when it was applied to microsensor construction. The modified electrode exhibited the classical features of a kinetically fast redox couple bound to an electrode surface and the redox potential of the redox polymer/enzyme film was 0.14 V (vs. SCE). For a scan rate of up to 200 mV s^-1, the peak-to-peak potential separation was less than 25 mV. In the presence of glucose, a typical catalytic oxidation current was observed, which reached a plateau at 0.25 V (vs. SCE). Under the optimal experimental conditions, the steady-state electrooxidation current measured at 0.30 V (vs. SCE) was linear to the glucose concentration in the range of 0 - 30 mM. Successful attempts were made in blood sample analysis.

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