Abstract − Analytical Sciences, 19(6), 957 (2003).
Spectrophotometric Determination of a Thiobarbituric Acid-Reactive Substance in Human Hair
Jenn-Yuan SHEU,* Po-Hua CHEN,** Wei-Chang TSENG,*** Chang-Yu CHEN,*** Li-Yu TSAI,** and Yeou-Lih HUANG*
*Graduate Institute of Medicine, Kaohsiung Medical University, Kaohsiung, Taiwan
**School of Technology for Medical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan
***Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan
**School of Technology for Medical Sciences, Kaohsiung Medical University, Kaohsiung, Taiwan
***Department of Medical Technology, Fooyin University, Kaohsiung, Taiwan
A simple and sensitive spectrophotometric method for the determination of a thiobarbituric acid-reactive substance (TBARS) in human hair has been developed. The proposed method is based on the formation of a red-colored product by the reaction of products of lipid peroxidation with thiobarbituric acid in an acidic medium. The absorbance of the resulting red product was measured at 534 nm. The linear dynamic range was between 1.0 and 20 µmol/L. The recoveries were 98.3 - 105.0%, and the relative standard deviations (RSD) were 0.32 - 1.24, respectively. TBARS in digested hair sample was stable for 3 days at room temperature. It was found that, using this method, the hair TBARS concentration in smokers (0.116 ± 0.030 µmol/g, n = 30) was significantly higher than that in non-smokers (0.096 ± 0.015 µmol/g, n = 30) (p < 0.05).
J-STAGE:
View this article in J-STAGE
Back to the Table of Contents
