Analytical Sciences

Flow Injection Analysis of Hydrogen Peroxide Using Glass-Beads Modified with Manganese(III)-Tetra(4-carboxyphenyl)porphine Derivative and Its Analytical Application to the Determination of Serum Glucose

Masaki MIFUNE,*  Katsuyoshi SUGIMOTO,* Akimasa IWADO,** Hiromichi AKIZAWA,** Noriko MOTOHASHI,*** and Yutaka SAITO*

*Faculty of Pharmaceutical Sciences, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan
**Graduate School of Sciences and Technology, Okayama University, Tsushima-Naka, Okayama 700-8530, Japan
***Kobe Pharmaceutical University, Motoyamakita-machi, Higashinada, Kobe 663-8179, Japan

A flow injection analysis system of hydrogen peroxide was developed. The present system is based on measuring of the absorbance of a quinoid dye formed by the reaction between aminoantipyrine and 2H₂O₂ (catalyzed by peroxidase), which produces quinoid dye and 4H₂O. A column packed with aminopropyl-glass beads modified with a manganese(III)-tetra(4-carboxyphenyl)porphine derivative (Mn-TCPP_G column), which has peroxidase-like activity, was used in place of an immobilized peroxidase column in the above reaction. The linear range of the calibration curve was 0.4 - 80 µg/ml hydrogen peroxide. The relative standard deviation of this system was 2.97% (n = 100, 10 µg/ml hydrogen peroxide 20 µl injection). The Mn-TCPP_G column has sufficiently stability for the continuous injection of hydrogen peroxide untill 100 times. The advantageous feature of the Mn-TCPP_G column was a less-electrostatic interaction between the mother glass beads and the anionic chromogen or quinoid dye formed and the stability in terms of the storage, temperature and moisture. The determination of serum glucose was achieved by attaching an immobilized glucose oxidase column to this system without deproteinization.

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